Real Time PCR Sistemleri

► Innovative 3D Hotlid Design 
► Integrated Tablet Computer, Convenient Operation,   Wireless Experience 
► 96 Wells High Throughput 
► Motor with Automatic Brake Function 
► Gradient Range 1°C to 36°C
 
Working Principle of the Machine
 
► The heating and cooling of the PCR block during  the PCR  process is done by an inbuilt Ferrotec peltier technology. 
► The LED excitation source in the system paired with advanced fiber optic transmission  technology makes the photoelectric
Detection system very sensitive and reliable. The fluorescent emission is detected by Photomultiplier Tube (PMT). 
► The Stepper motor with filters is driven to each and every well by a synchronous belt for systemic scanning of sample 
From different wells.

► The new model of Thermo Electric (TE) base plate (72 series) has a longer life span.
► The new adhesive technology used with the  advanced semi-conductor substrate:
► Improves the performance of the TE base plate   under highly humid conditions
► 
Greatly improves the life span of the TE base plate


Product Hardware

 
Innovative 3D Hot Lid is a new technology. It consists of a Pressure Box which exerts uniform pressure on the plate module through 6 compression springs. It gives perfect sealing and avoids sample evaporation or overloading due to lid imbalance. The Pressure Box consists of an outer plastic box which is embedded with a rubber pad. The rubber pad is covered by an aluminium plate. This plate fits snugly on the PCR plate with a certain amount of pressure. This airtight seal prevents the cold air and hot air connection on the module. Thus, the module bears dynamic temperature uniformity.

Unique PMT scanning system The detection probe at the bottom of the unique scanning device effectively prevents interference between two adjacent wells while reading a plate.

a) The probe has a long-life LED light source which   does not require maintenance.

b) Advanced optical fibre transmission technology and photoelectric detection system is extremely   sensitive and reliable.

c) Precise optical path system combined with sensitive PMT system detects fluorescence activity  accurately.


 
Product Name  Insta Q96™ Real-time PCR Detection System 
Product Code  LA1012
Sample Capacity  96-Well PCR plate, 12x8 Strip, 96x0.2ml (Bottom Transparent) 
Dynamic Range  1~1010 Copies 
Excitation Wavelength  300-800nm 
Emission Wavelength  500-800nm 
Detected Flourescence  F1: FAM, SYBR Green I; F2: VIC, HEX, TET,
JOE, CY3, NED, TAMRA; F3: ROX, TEXAS-RED; F4: CY5; F5:CY5.5 
Block Temp. Range  4~105°C (Minimum Increment 0.1°C) Soak Low
Temperature, Conservation Function 
Heating / Cooling Rate  4.0°C/s (max) 
Temp. Control Accuracy  ≤ ± 0.1°C 
Temp. Fluctuation  ≤ ± 0.1°C 
Temp. Uniformity  ≤ ± 0.3°C 
Temp. Control Mode  Block / Tube Simulation Mode (Automatic
Control Based On Sample Volume) 
Sample Volume Range  5~100µl 
Gradient Temp. Range  1~36°C 
Hot-Lid Temp. Range  30~110°C (Adjustable Default 105°C),
Automatic Hot-Lid 
Flourescence Detection Repeatability
 
Within 5%
Scan Mode  Entire Plate or Designated Line 
Program Max 20 Segments for each Program,
Max 99 Cycles 
Operation Mode  Continuous
Scan Period  5.5 seconds 
Feature Function Absolute Quantification, Relative Quantification,
HRM, SNP Analysis, Automatic Data Analysis,
Melt Curve,  Genotyping,  Gradient,  Automatic Gain,  
Background Correction,  Multi-Channel Crosstalk  Correction,  
Customized Parameters 
Operating System Microsoft: Windows 7/Windows 8.1/Windows 10,
Software: Excel 2000/2002/2003/2007/2012 
PC Configuration Memory: 512M, Hard Disk: 10GB,
CPU: Pentium 4, Virtual Memory: ≥ 1000MB 
 

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